Practical 7 : Propagation of freshwater macrophytes

INTRODUCTION

We were provided  with 3 types of freshwater macrophytes. Every group had to choose only one type which is  4 replicates each.

METHOD

Before proceed to the procedure, agar must be transfered first to allow them harden in time.

•         Excise root and leaf

•         Wash with sterile dH²O + tween 20 (3mins)

•         Rinse with sterile dH²O (3mins)

•         Wash with 70%,50%,30%,10% chlorox (1 min each)

•         Rinse with sterile dH²O  (3 mins)

•         In laminar flow,wash explant with 70% ethanol (3 mins)

•         Rinse with sterile dH²O  (3 mins)

•         Plant explants into agar

•         Observe and record